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KMID : 0380620170490040430
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Korean Journal of Food Science and Technology
2017 Volume.49 No. 4 p.430 ~ p.437
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Ameliorating effect of the ethyl acetate fraction of Pteridium aquilinum on glucose-induced neuronal apoptosis
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Park Seon-Kyeong
Guo Tian Jiao
Kim Jong-Min
Kang Jin-Yong
Park Sang-Hyun
Kang Jeong-Eun
Kwon Bong-Seok
Lee Chang-Jun
Lee Uk
Heo Ho-Jin
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Abstract
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The protective effect of Pteridium aquilinum on high glucose-induced cytotoxicity was examined in vitro to investigate the relationship between diabetic condition and neuronal dysfunction. The ethyl acetate fraction of P. aquilinum (EFPA), with total phenolic content of 265.08 mg gallic acid equivalent/g, showed higher 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)/2,2-diphenyl-1-picrylhydrazyl radical scavenging activities and lipid peroxidation inhibitory effect than any other fraction. In addition, EFPA showed a significant reduction in the inhibitory effect on α-glucosidase activity (IC50 value=205.26 μg/mL) compared to the acarbose positive control. The anti-oxidative effect in PC12 cells, protective effects on high glucose-induced oxidative stress in neuronal cells, and neurotoxicity were measured using 2',7'-dichlorofluorescin diacetate, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazoliumbromide, and lactate dehydrogenase assays, respectively. EFPA showed conspicuous inhibitory effect on cellular reactive oxygen species production and neuronal cell apoptosis. Finally, kaempferol-3-glucoside was identified as the main phenolic compound of EFPA using high performance liquid chromatography.
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KEYWORD
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α-glucosidase, antioxidant, kaempferol-3-glucoside, neuronal cell protection, Pteridium aquilinum
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